Reversible Gene Expression Control in Yersinia pestis by Using an Optimized CRISPR Interference System

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Optimized methods for biofilm analysis in Yersinia pestis.

Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system

Despite many methodological advances that have facilitated investigation of Mycobacterium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen remains difficult. Here, we describe an optimized CRISPR-based method to inhibit expression of essential genes based on the inducible expression of an enzymatically inactive Cas9 protein together with gene-specific...

Genotyping of Global Yersinia Pestis Isolates by Using Is285

Yersinia pestis is the etiologic agent of bubonic and pneumonic plague, one of the most dangerous bacterial infections. Plague is a re-emerging disease displaying current tendency to increasing reports of human cases, including the affliction with multidrug-resistant strains of Y. pestis. The plague bacterium is a potential agent of biowarfare and bioterrorism. Therefore, both military and civi...

Detection of a Yersinia pestis gene homologue in rodent samples

A homologue to a widely used genetic marker, pla, for Yersinia pestis has been identified in tissue samples of two species of rat (Rattus rattus and Rattus norvegicus) and of mice (Mus musculus and Apodemus sylvaticus) using a microarray based platform to screen for zoonotic pathogens of interest. Samples were from urban locations in the UK (Liverpool) and Canada (Vancouver). The results indica...